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Social Media links WEBSITE OF THE STATE OF SOUTH DAKOTA DEPARTMENT OF HEALTH
Doneen Hollingsworth, Secretary of Health
South Dakota Department of Health

Guidelines for Submitting Human Samples
for West Nile Virus and Other Arbovirus Testing

Serum

  1. Ideal timing of specimens is as follows:
    a) Acute serum should be collected 8-10 days after onset of symptoms. Acute
    serum samples collected too early after onset of symptoms may result in false negative results.
    b) Convalescent serum should be collected 2-3 weeks after the acute.
  2. Send 2-3 ml serum on cool packs. Serum separated from the red blood cells is
    preferred to prevent hemolysis.
  3. Standard blood collection tubes without anticoagulant should be used to collect
    the sample. (Red stopper, red/gray marble stopper, serum gel separator tubes)

Cerebrospinal Fluid

  1. Send 2-3 ml of Cerebrospinal fluid if available on cool packs. Do not freeze.
  2. Also send serum sample collected at the appropriate time. (see above)

Packaging and Shipping

  1. Send specimens properly packaged with absorbent material inside 2 plastic
    containers on cool packs.
  2. Specimens may be mailed or shipped by courier (i.e. UPS, Fed Ex). Areas
    that do not have next day mail service to Pierre should consider alternate
    shipping method.
  3. US Mail, UPS, and Federal Express all deliver samples to the SDPHL Monday
    through Friday only.
  4. Please call the laboratory at 773-3368 before shipping specimens so test plates
    can be prepared.

Additional Information to Send with Specimens

  1. Travel history (0-4 weeks before onset of symptoms).
  2. Vaccination history (Yellow fever, Dengue, Japanese Encephalitis)
  3. Date of symptom onset.
  4. Collection date.
  5. Physician contact information.

TEST CODE — WNV

CHARGE — Currently there is no charge to submitting agency.

RESULT REPORTING — Positive results will be phoned to the physician/submitting agency.

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